Generator

Part:BBa_K1688008:Design

Designed by: Fredrik Lindeberg   Group: iGEM15_Uppsala   (2015-09-15)


ModLac laccase with His-tag (inc RBS and J23110 promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 310
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene is followed by a KpnI restriction site with glycines and serines on each side, which are small amino acids which increase the flexibility of the protein. The gene was originally synthesized with a C-terminus export tag attached. This tag was removed through digestion with Kpn1 restriction enzyme and resulted in this sequence.


Source

D439A/M510L CueO mutant sequence received from Professor Kunishige Kataoka and synthesized with His-tag.

References